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Alpaca Anti-Rabbit IgG, Recombinant VHH (HRP)

Category: Nano-secondary antibody

Alpaca and Llama heavy chain only antibodies provide a framework for engineering recombinant antibodies. The monomeric variable domain (VHH) of heavy chain only camelid IgG2 and IgG3 provides a 12-15 kDa domain which is easy to clone and produce at high levels in recombinant systems, is stable to heat and pH extremes, and generally, has good solubility. Alpaca Anti-Rabbit IgG,Recombinant VHH (HRP) is an anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 3 anti-rabbit Nanobodies that bind to rabbit IgG FC with high affinity & specificity.

Product overview Documents References FAQ
  • Summary

Immunogen:Immunogen was Fc region of Recombinant Rabbit IgG

Specificity:This Recombinant single-domain antibody is specific to Rabbit IgG FC region

Isotype:VHH domain of alpaca IgG2a/2b

Clonality:mixture of 3 clones

Host:Alpaca pacous

Purity:Affinity purified

Affinity:VHH1=0.284nM VHH2=0.026nM VHH3<1pM

Cross Reacivty:No Cross-reactivity to human ,macaque mouse, rat,sheep,Goat and guinea pig IgG

Synonyms:Alpaca single domain antibody,VHH,Nanobody

Conjugate:HRP


  • Applications/Dilutions

Dilutions:Western Blot(WB) 1:100000 - 1:600000

NB001H are monovalent and high affinity antibodies that don’t cluster. Hence, they enable one-step WB: the simultaneous incubation of primary and secondary antibodies. This optional method saves incubation time and washing steps.

ELISA:1:5000-1:10000

Immunohistochemistry(IHC):1:500 - 1:1000

Immunoprecipitation(IP):1:500 - 1:5000


  • Packaging, Storage & Formulations

Storage:Store at -20℃

Buffer:Phosphate Buffered Saline (PBS) containing 0.2%BSA,50% glycerol

Preservative:0.1% Pro-Clean 400

Concentration:1。0 mg/ml

Purity:Recombinant Expression and Affinity purified

This product is for research use only and is not approved for use in humans or in clinical diagnosis.


资源 6.jpg


Fig1: Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBST for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Alpaca anti-rabbit IgG , recombinant VHH (HRP) (NB001H) was used for 1 hour at room temperature at 1:40,000 (Lane A, E), 1:80,000 (Lane B, F), 1:160,000 (Lane C, G) and 1:320,000 (Lane D, H).

Exposure time: 30 seconds

资源 8.jpg


Fig2: Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using rabbit anti-Cytokeratin 18 antibody.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200 dilution) for 30 minutes at room temperature. Alpaca anti-rabbit IgG, recombinant VHH (HRP) (NB001H, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/200 dilution。


资源 9.jpg

Fig3: Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8。0-8。4) for 20 minutes。The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature。 Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (NB001H, 1/200 dilution) was used for 30 min at room temperature。 Tissues were counterstained with hematoxylin and mounted with DPX。



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If you want to sell VHH on our platform, please contact us !

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13551086942

Email

service@nb-biolab.com

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